Hosted by Lucy Collinson
Lucy Collinson runs the Electron Microscopy Facility at the Francis Crick Institute in London. The facility works with over thirty research groups, imaging samples as diverse as proteins, DNA, cells, tissues and model organisms. The group uses light, electron and X-ray microscopes, and specialises in Correlative Light and EM (CLEM), Integrated Light and EM (ILEM), Serial Block Face SEM (SBF SEM), Focused Ion Beam SEM (FIB SEM), cryo soft X-ray tomography (SXY) and advanced image analysis.
Christopher Peddie is a Principal Scientific Officer in the Electron Microscope Facility at the Francis Crick Institute in London. From a background in central and peripheral neuroscience, he specialises in the 3-dimensional examination of cells and tissues using a variety of correlative light and electron microscopy techniques. He has established several automated serial sectioning instruments at the institute including the serial blockface scanning electron microscope (SBF SEM) and the automated tape-collection ultramicrotome (ATUMtome), and leads the continued development of the SECOM integrated light and scanning electron microscopy platform.
Matt Russell is a Senior Scientific Officer in the Electron Microscopy Facility at the Francis Crick Institute in London. He has been leading alpha testing of the Delphi microscope at the Facility, including testing sample preparation methods for Delphi imaging of whole cells. He is experienced in many of the techniques used in the facility, including the Phenom benchtop SEM, on which the Delphi is based.
Roland Fleck is the Director of the Centre for Ultrastructural Imaging at King’s College London. The centre supports applied and basic research in the life and physical sciences as well as providing a Diagnostic Electron Microscopy service to the NHS. The centre provides training and support in a wide range of electron microscopy techniques and procedures.
For the hands-on sessions there is a limited number of slots. The hands-on sessions will be guided by Matt Russell, Anne Weston and Christopher Peddie. We provide carousel sessions (groups will circulate), so everybody will do all 3 sessions:
A] Delphi, fully integrated CLEM; hands-on with test sample
B] Phenom, tabletop SEM; hands-on with test sample
C] Secom, integrated CLEM on Quanta SEM; hands-on with test sample
Anne Weston is a Senior Scientific Officer in the Electron Microscopy Facility at the Francis Crick Institute in London. She first started working in electron microscopy 19 years ago and works with both SEM and TEM with many of her SEM images being used within the scientific community and further afield commercially. She has experience in many of the techniques used in the facility including the Phenom benchtop SEM.